Common disinfection methods, applicable scenarios and operation points of transfer windows
\nThey are divided into three categories: daily physical disinfection, chemical wiping disinfection, and space fumigation sterilization. They are used separately in ordinary clean workshops and sterile\/biological workshops.
\n1. Ultraviolet disinfection (standard equipment, most transfer windows are equipped with it)
\nApplicable: Daily disinfection of material surfaces and interior of the box, standard for electronic interlock transfer windows
\nPrinciple: Short-wave UV-C damages the DNA of microorganisms, killing bacteria, molds and viruses
\nOperation guidelines
\nDo not block the lamp tube when placing materials, turn on the ultraviolet lamp after closing the door;
\nIn the ordinary clean area, the irradiation time is \u2265 15 minutes, in the sterile workshop \u2265 30 minutes;
\nAfter the irradiation is completed, turn off the ultraviolet lamp, and wait for 2-3 minutes before opening the door. Notes for Attention
\nWhen the ultraviolet light is on, do not touch or look directly at the lamp tube;
\nIf the lamp tube accumulates dust, it will significantly reduce the sterilization effect. Clean it weekly;
\nAfter a cumulative usage of 800 hours, the lamp tube must be replaced. The irradiation intensity must be lower than 50\u03bcW\/cm\u00b2 for failure.
\nLimitations: There are shadow dead zones that cannot penetrate the packaging. It only provides surface sterilization.
\nII. Chemical wiping disinfection (pre-treatment of incoming and outgoing materials, daily cleaning of the box)
\nThe most basic and daily routine in the workshop, there are two commonly used disinfectants
\n1. 75% medical ethanol (alcohol)
\nUsage: Rapid disinfection of plastics, stainless steel, glass, and outer packaging surfaces;
\nAdvantages: Fast evaporation, no residue, does not damage stainless steel;
\nPrecautions: Do not spray in large areas for a long time, and there is a risk of fire when exposed to ultraviolet or electric sparks.
\n2. 0.2% – 0.5% peracetic acid
\nUsage: High-level disinfection in sterile workshops, biological laboratories, etc., with stronger sterilization power;
\nNote: It has a slight corrosive property, wipe it with a cloth, let it stand for 5 minutes before wiping clean with pure water, to avoid long-term corrosion of the sealing strip.
\nOperation process
\nRemove the outer packaging of the materials in the outer area \u2192 Dip the dust-free cloth in the disinfectant and wipe all six sides of the materials \u2192 Dry and put them in the transfer window; At the end of each shift, wipe the inner wall, door frame, sealing strip, and glass of the box.
\nIII. Hydrogen peroxide vaporization \/ atomization disinfection (VHP, dedicated for high-clean and sterile areas)
\nApplicable: Sterile pharmaceuticals, cell rooms, biological safety supporting transfer windows, regular thorough sterilization
\nAdvantages: No dead zones, better penetration than ultraviolet, can kill spores;
\nUsage scenarios: Deep sterilization every week\/month, after major equipment maintenance, or after contamination risk;
\nOperation: Close the double doors, start the atomization device, maintain a certain concentration for 30-60 minutes, complete ventilation replacement before use;
\nSupplementary: Some laminar transfer windows can be equipped with an atomization interface for batch space sterilization.
\nIV. Ozone disinfection (used in old-fashioned \/ simple clean rooms, gradually being phased out)
\nPrinciple: Strong oxidation to kill microorganisms;
\nDisadvantages: Corrodes rubber sealing strips, stainless steel turns yellow over time, has a strong irritant, requires long ventilation time;
\nUsage: Only for emergency deep disinfection, close for 20 minutes, open the door for at least 30 minutes for ventilation before operation;
\nCurrent situation: Sterile workshops are not recommended, VHP is preferred as a replacement.
\nV. Self-cleaning air blow-off disinfection (exclusive for laminar transfer windows)
\nSelf-cleaning transfer window with FFU high-efficiency filtration as a supporting means:
\nClose the door, turn on the fan, and perform one-way laminar air blowing for 10-20 minutes;
\nContinuous removal of dust and floating bacteria through the high-efficiency filter;
\nUsually used in combination with ultraviolet, first blow off and then ultraviolet sterilization for double protection.
\nVI. High-temperature disinfection (rarely used, only for heat-resistant metal tools)
\nOnly stainless steel tools and molds can be heated for a short time; Plastic, rubber sealing strips, and lamp tubes are strictly prohibited from high temperatures, as they will accelerate aging and deformation.
\nDisinfection combination schemes for different scenarios
\nElectronic cleanroom (10,000-level \/ 100,000-level)
\nEvery day: 75% alcohol wipe of materials + ultraviolet irradiation for 15 minutes; Weekly overall alcohol wipe of the box.
\nSterile pharmaceutical, medical device clean area
\nMaterial pre-treatment alcohol wipe \u2192 Ultraviolet for 30 minutes; Once a week, deep hydrogen peroxide vaporization sterilization.
\nBiological laboratory, positive control area
\nOuter layer disinfection + VHP space sterilization regularly, ultraviolet as auxiliary daily.
\nDisinfection key points
\nDisinfection must be carried out in a state where the double doors are completely closed, and opening for disinfection is prohibited;
\nDisinfectants should avoid high-concentration strong acids and strong alkalis to prevent corrosion of the box and sealing strips;
\nUltraviolet is only for surface disinfection, gaps, folds, and shaded areas need to be wiped together;
\nAfter vaporization \/ fumigation, sufficient ventilation is required, as residual disinfectants will be brought into the clean area and cause pollution.<\/p>","protected":false},"excerpt":{"rendered":"
Common disinfection methods, applicable scenarios and operation points of transfer windows They are divided into three categories: daily physical disinfection, chemical wiping disinfection, and space fumigation sterilization. They are used separately in ordinary clean workshops and sterile\/biological workshops. 1. Ultraviolet disinfection (standard equipment, most transfer windows are equipped with it) Applicable: Daily disinfection of material […]<\/p>","protected":false},"author":12,"featured_media":4884,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":{"_acf_changed":false,"footnotes":""},"categories":[92],"tags":[],"class_list":["post-5532","post","type-post","status-publish","format-standard","has-post-thumbnail","hentry","category-industry-technology"],"acf":[],"_links":{"self":[{"href":"https:\/\/www.bacintl.com\/es\/wp-json\/wp\/v2\/posts\/5532","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/www.bacintl.com\/es\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/www.bacintl.com\/es\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/www.bacintl.com\/es\/wp-json\/wp\/v2\/users\/12"}],"replies":[{"embeddable":true,"href":"https:\/\/www.bacintl.com\/es\/wp-json\/wp\/v2\/comments?post=5532"}],"version-history":[{"count":1,"href":"https:\/\/www.bacintl.com\/es\/wp-json\/wp\/v2\/posts\/5532\/revisions"}],"predecessor-version":[{"id":5533,"href":"https:\/\/www.bacintl.com\/es\/wp-json\/wp\/v2\/posts\/5532\/revisions\/5533"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/www.bacintl.com\/es\/wp-json\/wp\/v2\/media\/4884"}],"wp:attachment":[{"href":"https:\/\/www.bacintl.com\/es\/wp-json\/wp\/v2\/media?parent=5532"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/www.bacintl.com\/es\/wp-json\/wp\/v2\/categories?post=5532"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/www.bacintl.com\/es\/wp-json\/wp\/v2\/tags?post=5532"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}